D2000
DNA ladder
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貨號(hào)
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規(guī)格
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名稱
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RTM415-02
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100次(2×250 μl)
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D2000 DNA
ladder
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● 產(chǎn)品簡介：
本產(chǎn)品是由6條雙鏈DNA條帶組成，適用于瓊脂糖凝膠電泳中DNA條帶的分析。產(chǎn)品中已含有1×loading
buffer，可根據(jù)實(shí)驗(yàn)需要，直接取2-5 μl電泳，使用方便，電泳圖像清晰。6條帶的大小分別為100，250，500，750，1000，2000
bp。其中750 bp條帶濃度約為100 ng/5 μl，顯示加亮帶，其余條帶濃度約為50 ng/ 5μl。
● 貯存、效期及運(yùn)輸：
-20℃貯存；有效期3年；濕冰運(yùn)輸。
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使用方法：
1. 取2-5 μl本產(chǎn)品加入到瓊脂糖凝膠的加樣孔中（每1 mm×1 mm加樣孔加1 μl，如果加樣孔
寬于5 mm，可以適當(dāng)增加上樣量）就行電泳。
2. 建議電泳條件：凝膠濃度為1.0-2.0％，凝膠長度5-7
cm，電泳電壓4-10 v/cm，電泳時(shí)間15-20分鐘。
3. 通過核酸染料染色后紫外燈或藍(lán)光儀觀察條帶。
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注意事項(xiàng)：
1. 瓊脂糖的質(zhì)量對(duì)DNA的電泳有很大影響，電泳時(shí)請(qǐng)盡量使用質(zhì)量優(yōu)等的瓊脂糖。
2. 由于PAGE電泳的特殊性，該產(chǎn)品不建議用于PAGE電泳。
3. 請(qǐng)使用新鮮配制的電泳緩沖液和新鮮配制的凝膠進(jìn)行電泳，以保證Marker良好的分離效果。
4.
該Marker適用于DNA片段大小的確定和對(duì)DNA含量的粗略定量，不適用于DNA片段含量的精確定量。
5．推薦使用RealSafe系列染料（貨號(hào)：GR001，GR002，GR003，GR004）染色。如果使用Goldview（貨號(hào)：GV001）或EB（貨號(hào)：EB001）等染料進(jìn)行染色，由于此類染料帶更多的正電荷（染料移動(dòng)方向與核酸泳動(dòng)方向相反），隨著電泳時(shí)間延長，染料向大片段聚集，凝膠會(huì)出現(xiàn)陰陽膠現(xiàn)象（染料含量高的凝膠紫外下較亮，含量低的凝膠紫外下發(fā)暗），導(dǎo)致小分子
量DNA顯色很弱或不可見。陰陽膠可以在電泳結(jié)束后浸泡染色，將膠浸沒在含EB或Goldview的電泳緩沖液中搖動(dòng)染色20-25
min，小分子量DNA會(huì)顯色清楚。更長時(shí)間浸泡會(huì)因DNA分子擴(kuò)散而使條帶彌散，小分子量DNA更易彌散。
6．紫外照射下EB易分解，使DNA條帶熒光變淺。DNA受紫外光照射形成嘧啶二聚體，不利于待回收片段的下游工作，因此盡可能減少紫外照射時(shí)間。
7．蔗糖會(huì)結(jié)合DNA，降低其電泳遷移率，建議DNA樣品上樣使用含甘油的上樣緩沖液，如6×DNA上樣緩沖液（雙染料）（貨號(hào)：DL020）。
8．大部分核酸工具酶會(huì)結(jié)合DNA，降低其電泳遷移率，大分子量DNA尤為明顯，建議此類DNA樣品上樣使用含SDS的上樣緩沖液，如6×DNA上樣緩沖液（含SDS）（貨號(hào)：DL090）。
使用RTM415 D2000 DNA ladder產(chǎn)品發(fā)表部分文章列表：
1. [2021 IF=20.37] A multi-locus phylogenetic
evaluation of Diaporthe (Phomopsis)
Marker :RTM415，D2000
DNA ladder
Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W.
Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde
Journal: Fungal Diversity (2012)
56:157–171
Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese
Academy of Sciences
Paper link：https://link.springer.com/article/10.1007/s13225-012-0190-9
2. [2021 IF=1.17] Rhopalostroma
brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.
Marker :RTM415，D2000
DNA ladder
Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG,
MARC STADLER，ALI H.BAHKALI, KEVIN D.
HYDE
Journal: Phytotaxa，2015，227
(3): 229–242
Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese
Academy of Sciences
Paper link：https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.227.3.2/0
3. [2021 IF=3.24] Envelope Proteins ofWhite
Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like
Protein (LvPT).
Marker :RTM415，D2000
DNA ladder
Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang
Journal: Plos One，Published: December
21, 2015
Institution：Key Laboratory of Experimental Marine Biology, Institute of Oceanology,
Chinese Academy of Sciences
Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144922
4. [2021 IF=2.88] Genetic diversity and
genetic structure of Decalobanthus
boisianus in Hainan Island, China.
Marker :RTM415，D2000 DNA ladder
Author: Huan Jiang，Wenxing Long， Hui Zhang，Chengneng Mi，Tao Zhou，Zongzhu Chen
Journal: Ecology and Evolotion Volume
569, 15 March 2019, Pages 28-30
Institution：Institute of Tropical Agriculture and Forestry, Hainan University
Paper
link： https://doi.org/10.1002/ece3.5127
5. [2021 IF=1.17] Dendrostoma covidicola sp.
nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province,
China.
Author: MILAN C. SAMARAKOON, ITTHAYAKORN
PROMPUTTHA & JIAN-KUI (JACK) LI
Marker: RTM415，D2000
DNA ladder
Journal: Phytotaxa. 2021，483 (2): 085–094.
Institution：University of Electronic Science and
Technology of China
Paper link：https://doi.org/10.11646/phytotaxa.483.2.1
6. [2021 IF=2.6] Natural occurrence
of Alternaria mycotoxins in wheat and potential of reducing
associated risks using magnolol.
Marker :RTM415，D2000 DNA ladder
Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan
Jiang,Yunchao Li and Meng Wang
Journal: Journal of the Science
of Food and Agriculture Volume101,
Issue7，2021，3071-3077
Institution：Beijing Research Center for Agricultural Standards and Testing, Beijing
Academy of Agriculture and Forestry Sciences
Paper
link：https://onlinelibrary.wiley.com/doi/10.1002/jsfa.10901
7. [2021 IF=20.37] Taxonomy, Phylogeny,
Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae
(Sordariomycetes)
Marker :RTM415，D2000 DNA ladder
Author: Kevin
D Hyde，Jian-Kui Liu
Journal: Fungal Diversity volume
112, pages 1–88 (2022)
Institution：School of Life Science and Technology, Center for Informational Biology,
University of Electronic Science and Technology of China
Paper
link：https://link.springer.com/article/10.1007/s13225-021-00495-5
8. [2022 IF=8.4] Target and acidic tumor
microenvironment-induced in situ HCR for bispecific tumor cell imaging and
therapy.
Marker :RTM415，D2000 DNA ladder
Author: Zihan
Song, Yuzhe Fan, Su Zeng, Lianli Sun
Journal: Sensors and Actuators: B. Chemical 413 (2024) 135867
Institution：College of Pharmaceutical Sciences, Zhejiang University
Paper
link：https://doi.org/10.1016/j.snb.2024.135867
9. [2023 IF=14.3] Bioactive Self-Assembled
Nanoregulator Enhances Hematoma Resolution and Inhibits Neuroin?ammation in the
Treatment of Intracerebral Hemorrhage.
Marker :RTM415，D2000 DNA ladder
Author: Wenyan
Yu, Chengyuan Che, Yi Yang, Yuzhen Zhao, Junjie Liu, Aibing Chen,and Jinjin Shi
Journal: Adv. Sci. 2024,
2408647
Institution：School of Pharmaceutical Sciences，Zhengzhou
University
Paper
link：https://doi.org/10.1002/advs.202408647